ERK Activation by Retinal Progenitor Cells Protect RPE Cells from Oxidative Damage
نویسندگان
چکیده
Age-related macular degeneration (AMD) results from oxidative damage to the retinal pigment epithelium (RPE) with concomitant photoreceptor cell death. The effects of oxidative stress were investigated on a human RPE cell line (ARPE-19) in the presence of 48h-conditioned media from human retinal progenitor cells (RPC). Freshly isolated RPCs rapidly formed spherical clusters (neurospheres) and expressed markers of undifferentiated retinal progenitor cells such as nestin and pax6. Conditioned media from RPC neurospheres inhibited RPE cell death and upregulated ERK 1/2 activity. The protective effect of RPC-CM was blocked by U0126, a selective inhibitor of MEK1/2. Intravitreal injections of RPC-CM suppressed photoreceptor cell death in dystrophic rat retinas. Our study shows that adult human retinal progenitor cells secrete putative prosurvival molecules that protect RPE cells from oxidative stress and suppress photoreceptor cell death in vivo. Trophic factors secreted by human RPCs may prove useful in the treatment of degenerative retinal diseases such as AMD.
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